Chapter1 Basic Knowledge of Analytical Chemistry Experiments1
1.1Tasks and Requirements of Analytical Chemistry Experiment1
1.2Laboratory Safety Knowledge2
1.3Basic Knowledge of Quantitative Analysis Experiments2
1.4Instruments and Basic Operations of Quantitative Analysis6
1.5Recording,Processing and Reporting of Experimental Data15
Chapter2 Sample Preparation and Separation Techniques20
2.1Preparation of Samples20
2.2Separation Techniques of Samples20
Chapter3 Basic Experiments for Quantitative Analysis29
Experiment 3.1Analytical Balance Weighing Exercise29
Experiment 3.2Basic Operation for Titration Analysis30
Experiment 3.3Calibration of Volumetric Instruments33
Experiment 3.4Preparation and Standardization of HCl and NaOH Solution35
Experiment 3.5Determination of Total Acidity in Edible Vinegar39
Experiment 3.6Determination of Nitrogen Content in Ammonium Salts—Formaldehyde Method41
Experiment 3.7Determination of Mixed Alkali—Double Indicator Method44
Experiment 3.8Preparation and Standardization of EDTA Solution47
Experiment 3.9Determination of Total Hardness of Tap Water50
Experiment 3.10Determination of Aluminum Content in Industrial Aluminum Sulfate53
Experiment 3.11Determination of Zn2+ and Bi3+ in Mixed Solution by Continuous Titration55
Experiment 3.12Preparation and Standardization of Potassium Permanganate Solution58
Experiment 3.13Determination of H2O2 Content in Hydrogen Peroxide Solution61
Experiment 3.14Determination of Iron in Iron Ore by Potassium Dichromate Method63
Experiment 3.15Preparation and Standardization of Iodine and Sodium Thiosulfate66
Experiment 3.16Determination of Glucose in Glucose Oral Solution72
Experiment 3.17Determination of Copper Content in Copper Alloys75
Experiment 3.18Determination of Chlorine in Tap Water by Mohr Method77
Experiment 3.19Determination of Nickel in Alloy Steel by Dimethylglyoxime Gravimetric Method79
Experiment 3.20Determination of Trace Iron with Phenanthroline by UV-Vis Spectroscopy82
Experiment 3.21Potentiometric Titration of Phosphoric Acid85
Chapter4 Comprehensive Design Experiments90
4.1Chemistry90
Experiment 4.1Determination of Benzoic Acid Content90
Experiment 4.2Determination of Phenol Content92
Experiment 4.3Determination of Purity of Soluble Barium Salt by Gravimetric Method95
Experiment 4.4Determination of Methanol Content in Industrial Alcohol98
Experiment 4.5Determination of Acetic Acid Content by Potentiometric Titration101
Experiment 4.6Determination of Iron，Aluminum，Calcium and Magnesium in Cement Clinker104
4.2Environment109
Experiment 4.7Determination of Total Alkalinity of Water109
Experiment 4.8Determination of Chemical Oxygen Demand（COD）in River Water111
Experiment 4.9Determination of Sulfate Content in Urban Sewage114
Experiment 4.10Determination of Formaldehyde in Waterborne Coatings by Spectrophotometry116
Experiment 4.11Determination of Glyphosate Content in Roundup120
4.3Biology121
Experiment 4.12Determination of Total Nitrogen Content for Compound Fertilizers121
Experiment 4.13Determination of Soil Organic Matter Content124
Experiment 4.14Determination of Chlorine Content for Compound Fertilizers127
Experiment 4.15Determination of Potassium Content for Compound Fertilizers 130
Experiment 4.16Determination of Available Phosphorus Content for Compound Fertilizers132
Experiment 4.17Determination of Available Phosphorus in Soil134
Experiment 4.18Macroporous Resin Adsorption and Spectrophotometric Determination of Flavonoids from Gingko Leaves137
Experiment 4.19Determination of Mixed Amino Acids Content 140
Experiment 4.20Determination of Reducing Sugar in Tobacco Leaf 142
4.4Pharmacy144
Experiment 4.21Determination of NH+4 in Chinese Medicine White Sal-Ammoniac 144
Experiment 4.22Determination of Calcium in Calcium Supplement by KMnO4 Method147
Experiment 4.23Determination of Al3+ and Mg2+ in Compound Aluminium Hydroxide Tablets150
Experiment 4.24Determination of the Content of Berberine Hydrochloride154
Experiment 4.25Determination of Acetaminophen in Ankahuangmin Capsules156
4.5Food158
Experiment 4.26Determination of Citric Acid Content in Commercially Available Citrus158
Experiment 4.27Determination of Calcium Content in Calcium Tablets/Milk Powder/Spinach161
Experiment 4.28Determination of NaCl Content in Soy Sauce163
Experiment 4.29Determination of Anthocyanin Content in Blueberries165
Experiment 4.30Determination of Melamine in Milk Products167
Experiment 4.31Determination of Vitamin B12 in Health Foods169
Chapter5 Innovation Experiments172
5.1Chemistry Experiment Competition for College Students172
Experiment 5.1Preparation of Rare Earth Europium（Eu） Complex and Determination of Coordination RatFio172
Experiment 5.2Preparation of Iron Oxide Nanoparticles and Its Application in the Determination of Melamine in Dairy Products175
Experiment 5.3Study on Synthesis of Complex\\\\\\\\[Ni（Me3en）（acac）\\\\\\\\]BPh4 and Its Solvent/Thermochromic Behavior177
Experiment 5.4Determination of Copper Content in Polynuclear Copper（Ⅰ） Complex180
5.2Research Training Experiments182
Experiment 5.5Gemini Ionic Liquid Modified Graphene Oxide Membranes for ReO-4/TcO-4Adsorption182
Experiment 5.6Colorimetric Determination of Uric Acid with Gold Nanoparticles183
Experiment 5.7A Green Chemistry Technique for Dissolution and Recovery of Gold from Electronic Wastes184
Experiment 5.8Extraction，Content Analysis and Cosmetic Application of Natural Pigment Betacyanin185
Appendix189
Appendix1Concentrations and Densities of Common Acid-Base Solutions189
Appendix2Common Primary Standard Substances and Their Drying Conditions and Applications189
Appendix3Preparation of Common Buffer190
Appendix4Common Indicators193
Appendix5Dissociation Constants of Weak Acids and Bases194
Appendix6Stability Constants of Coordination Compounds Formed by Metal Ions and EDTA（18-25℃，I=0.1 mol/L）198
Appendix7Standard Atomic Weights（1995，IUPAC）198
第1章分析化学实验基础知识200
1.1分析化学实验的任务和要求200
1.2实验室安全知识200
1.3定量分析实验基础知识201
1.4定量分析实验仪器及基本操作203
1.5实验数据的记录、处理和实验报告210
第2章常用样品制备及分离技术214
2.1样品的制备214
2.2样品的分离214
第3章定量分析基础实验220
实验3.1分析天平的称量练习220
实验3.2滴定分析的基本操作练习——酸碱滴定221
实验3.3容量仪器的校准223
实验3.4酸碱溶液的配制与标定225
实验3.5食用醋中总酸度的测定228
实验3.6铵盐中氮含量的分析——甲醛法230
实验3.7混合碱的分析——双指示剂法232
实验3.8EDTA溶液的配制与标定234
实验3.9自来水硬度的测定236
实验3.10工业硫酸铝中铝含量的分析238
实验3.11锌铋混合溶液中Zn2+、Bi3+含量的连续测定240
实验3.12高锰酸钾标准溶液的配制与标定243
实验3.13双氧水中过氧化氢含量的测定245
实验3.14铁矿石中铁含量的测定247
实验3.15碘和硫代硫酸钠溶液的配制与标定249
实验3.16葡萄糖口服液中葡萄糖含量的测定254
实验3.17铜合金中铜含量的测定256
实验3.18莫尔法测定自来水中的氯258
实验3.19丁二酮肟重量法测定合金钢中的镍260
实验3.20邻二氮菲分光光度法测定微量铁262
实验3.21磷酸的电位滴定265
第4章综合设计性实验269
4.1化学类269
实验4.1苯甲酸含量的测定269
实验4.2苯酚含量的测定271
实验4.3重量法测定可溶性钡盐的纯度273
实验4.4工业酒精中甲醇含量的测定275
实验4.5电位滴定法测定醋酸含量278
实验4.6水泥熟料中铁、铝、钙、镁含量的测定280
4.2环境类284
实验4.7水质总碱度测定284
实验4.8河水中化学需氧量（COD）的测定286
实验4.9城市污水中硫酸盐含量的测定288
实验4.10分光光度法测定水性涂料中的甲醛290
实验4.11农达中草甘膦含量的测定293
4.3生物类294
实验4.12复合肥料中总氮含量的测定294
实验4.13土壤中有机质含量的测定297
实验4.14复合肥料中氯离子含量的测定299
实验4.15复合肥料中钾含量的测定301
实验4.16复合肥料中有效磷含量的测定302
实验4.17土壤中有效磷的测定304
实验4.18银杏叶总黄酮含量的测定306
实验4.19混合氨基酸的测定309
实验4.20烟叶样品中还原糖的测定310
4.4制药类312
实验4.21中药白硇砂中NH+4含量的测定312
实验4.22高锰酸钾法测定补钙剂中钙含量314
实验4.23复方氢氧化铝片中Al3+和Mg2+含量的测定317
实验4.24盐酸黄连素含量的测定320
实验4.25氨咖黄敏胶囊中对乙酰氨基酚含量的测定321
4.5食品类323
实验4.26市售柑橘中柠檬酸含量的测定323
实验4.27钙片/奶粉/菠菜中钙含量的测定325
实验4.28酱油中NaCl含量的测定327
实验4.29蓝莓中花青素含量的测定329
实验4.30奶制品中三聚氰胺的测定331
实验4.31保健食品中维生素B12的测定332
第5章探索创新实验335
5.1大学生化学竞赛（分析化学部分）335
实验5.1稀土铕配合物的制备及配位比的测定335
实验5.2氧化铁纳米颗粒的制备及用于奶制品中三聚氰胺的测定337
实验5.3配合物\\\\\\\\[Ni(Me3en)(acac)\\\\\\\\]BPh4的合成及其溶剂/热致变色行为研究339
实验5.4多核铜(Ⅰ)配合物中铜含量的测定341
5.2创新性实验342
实验5.5Gemini离子液体改性氧化石墨烯薄膜在ReO-4/TcO-4吸附中的应用342
实验5.6金纳米粒子比色法检测尿酸343
实验5.7电子废弃物中金元素的绿色溶解与提取344
实验5.8天然色素甜菜红的提取、含量分析及其美妆应用344
附录349
附录1常用酸碱的密度、含量和浓度349
附录2常用基准物质的干燥条件和应用349
附录3常见缓冲溶液的配制350
附录4常用酸碱指示剂及配制方法352
附录5弱酸、弱碱的解离常数352
附录6金属离子与EDTA生成配位化合物的稳定常数（18～25℃，I=0.1mol/L）356
附录7原子表（1995，IUPAC）357
参考文献358
