Chapter 1 Overview and Prospects of Biopharmaceutics
New peptide and protein drugs(新型多肽及蛋白质类药物)
Antisense technology and antisense therapeutics(反义RNA与反义药物)
DNA Drugs
Antibodies and genetically engineered related molecules
1.1 Development of genetically engineered pharmaceutials(基因工程药物发展概况)
1.1.1 A brief history of the development of the world biopharmaceutical industry and current situation(世界生物制药产业发展简史与现状)
1.1.2 Development of genetic engineering drugs in Chma(我国基因工程药物的发展)
1.1.3 Characteristics of genetically engineered pharmaceuticals production(基因工程制药的特点)
1.1.4 Genetically engineered pharmaceuticals and human genome research and post genome research(基因工程药物与人类基因组、后基因组研究)
1.2 Development trend ofgenetically engineered pharmaceuticals(基因工程药物发展趋势)
1.2.1 New mode of research&development ofnew type biopharmaceuticals(研发新型生物药物的新模式)
1.2.2 Human genome project and~search and development ofnovel genetically engineered pharmaceuticals(人类基因组计划与基因工程新药的研发)
1.2.3 Discovery of drug target natural ligand and development ofnew drug(药物靶标天然配基的发现与新药的研发)
1.2.3.1 Mass spectrometry(质谱)
1.2.3.2 Two-hybridscreening(双杂交系统)
1.2.3.3 Phage display(展示技术)
1.2.4 Construction ofbiomolecule libraries in the hope ofsearching out new drugs(构建生物分子库以发现新药)
1.2.4.1 Site-directedmutagenesis(定点突变)
1.2.4.2 DNA shuffling(DNA洗牌技术)
1.2.4.3 Rationaldrugdesign orcomputer-aideddrugdesign(合理药物设计或计算机辅助药物设计)
1.2.5 Protein engineering and new drug research(蛋白质工程与新药研究)
1.2.5.1 lnsulin analog(新型胰岛素)
1.2.5.2 Creation ofnovelenzymes bymutagenesis(通过突变获得新型酶)
1.2.6 Glycosylation engineering and new drug research(糖基化工程与新药研究)
1.2.7 Metabolic engineering-combinatorial biology and new drug R&D(代谢工程-组合生物学与新药研发)
1.2.8 Research onnovelbiopharmaceuticalpreparations(新型生物药物制剂的研究)
Chapter 2 Basic Principles of Genetic Engineering
2.1 The definition and history ofgenetic engineering(基因工程的定义及发展沿革)
2.1.1 The definition of Genetic engineering(基因工程的定义)
2.1.2 History of genetic engineering(基因工程的发展沿革)
2.1.2.1 Bacterial transformation(细茵转化实验)(Avery,MaeLeod,McCarty)1940s
2.1.2.2 Hershey-Chase experimentonphageinfection(噬茵体感染实验)(Hershey,Chase)1952
2.1.2.3 WatsonandCrick-doublehelixmodelofDNA(Waston-CriekDNA双螺旋模型)(1953)
2.1.2.4 Successfully decoding ofthe genetic code(遗传密码的成功破译)
2.1.2.5 Lac operon of the model bacterium Escherichia coli(Francois Jacob，David Perrin,Carmen Sanchez and Jacques Monod)(模式细菌大肠埃希茵的乳糖操纵子)(1960)
2.1.2.6 The discovery of restrien'on enzymes andthe modification enzyme(限制酶与修饰酶的发现)(Werner Arber)(1962)
2.1.2.7 Isolation of enzymeDNA ligase(DNA连接酶的分离)(1967)
2.1.2.8 Discovery of reverse transcriptase(逆转录酶的发现)(Howard Temin，David Baltimore)(1970)
2.1.2.9 Methods for sequencingDNA(DNA测序方法)(Walter Gilbert and Frederick Sanger)(1977)
2.1.2.10 Discovery of plasmids(质粒的发现)
2.1.2.11 1stsuccessful recombination and expression Of DNA(DNA的首次成功重组与表达)(Paul Berg,Stanley Norman Cohen)1972—1973
2.1.2.12 Development ofp olymerase chain reaction(PCR)(聚合酶链反应技术的发展)(Kary Mullis)1983—1986
2.1.3 Applications of genetic engineering(基因工程的应用)
2.2 The main research content of genetic engineering(基因工程的主要研究内容)
2.2.1 Acquiringtargetgenes carrymggeneticinformation(获得具有遗传信息的目的基因)
2.2.1.1 Shotgun cloning(鸟枪克隆法)
2.2.1.2 Artificialsynthesis oftargetgene(人工合成目的基因)
2.2.1.3 Newapproachesfo，screeninggenes interested(筛选目的基因的新方法)
