光散射技术及其在生化药物分析中的应用

光散射现象在自然界中广泛存在，光散射分析化学是近年来的一个热点研究领域，已成为分析科学的重要分支。本书主要介绍了光散射分析技术的方法、原理及其在生物大分子、有机小分子和药物中的分析应用。
本书可作为从事分子光谱分析工作的研究生以及相关领域科研人员的参考书。

    

《光散射技术及其在生化药物分析中的应用》介绍了光散射分析技术的方法、原理及其在生物大分子、有机小分子和药物中的分析应用，散射光产生于光子与微小粒子的相互作用。当光子作用于任何不均匀介质时，都有散射光产生。结合激光技术，散射光已在胶体化学、高分子化学等粒子大小表征和分子量测定方面得到了应用，但在吸收和发射（荧光）光谱分析中，散射光却是严重的干扰因素。1993年，美国学者Pasternack及其合作者发现使用普通荧光光度计获得的光散射信号可应用于生物色素聚集体研究。在此基础上，我们把普通荧光光度计测定的光散射信号与物质的量联系起来，建立了以散射发光为检测信号的光谱分析法。近十年来，基于散射光信号的光谱分析法已为分析工作者广泛应用于无机离子、蛋白质和核酸、药物的9n,4定中，发展十分迅速，并随着纳米科学的发展取得了新成就。作为近年来工作的总结，我们选编了本论文集。内容主要涉及到我攻读博士学位期间完成的工作和我在西南大学所指导的研究生的论文。在此，感谢我的恩师北京大学童沈阳教授及其课题组的李克安教授、刘锋教授、赵凤林教授和李娜教授等的指导和帮助，感谢我的研究生们近年来为光散射分析所付出的努力，并希望他们在今后的工作中取得优异成绩。

Chapter 1 Developing Process of Light Scattering Technique Analysis
1.1 Resonance Light Scattering Technique Used for Biochemical and Pharmaceutical Analysis
1.2 The Principles and Analytical Applications of Total Internal Reflected Resonance Light
Scattering Technique
1.3 Recent Developments of the Resonance Light Scattering Technique: Technical Evolution,
New Probes and Applications
Chapter 2 Analytical Applications in DNA Detection of Light Scattering Technique
2.1 Determination of Nucleic Acids by a Resonance Light-scattering Technique
with α,β,γ,δ-tetrakis [4-(trimethylammoniumyl)phenyl]Porphine
2.2 Hybridization Detection of DNA by Measuring Organic Small Molecule Amplified Resonance
Light Scattering Signals
2.3 Determination of Nanograms of Nucleic Acids by their Enhancement Effect on the Resonance
Light Scattering of the Cobalt(II)/4-[(5-chloro -2-pyridyl) azo]- 1,3-diaminobenzene complex
2.4 Interactions of Janus Green B with Double Stranded DNA and the Determination of DNA
Dased on the Measurement of Enhanced Resonance Light Scattering
2.5 A Sensitive and Selective Assay of Nucleic Acids by Measuring Enhanced Total Internal
Reflected Resonance Light Scattering Signals Deriving from the Evanescent Field
at the Water/Tetrachloromethane Interface
2.6 Backscattering Light Detection of Nucleic Acids with Tetraphenylporphyrin-Al(III)-Nucleic
Acids at Liquid/Liquid Interface
2.7 Directly Light Scattering Imaging of the Aggregations of Biopolymer Bound Chromium(III)
Hydrolytic Oligomers in Aqueous Phase and Liquid/Liquid Interface
Chapter 3 Analytical Applications in Protein Detection of Light Scattering Technique
3.1 Determination of Protein Concentration by Enhancement of the Preresonance Light-scattering
of α,β,γ,δ-tetrakis(5-sulfothienyl) Porphine
3.2 On the Factors Affecting the Enhanced Resonance Light Scattering Signals of the Interactions
Between Proteins and Multiply Negatively Charged Chromophores Using Water Blue as an Example
3.3 Determination of Proteins with α,β,γ,δ-tetrakis(4-sulfophenyl) Porphine by Measuring
the Enhanced Resonance Light Scattering at the Air/Liquid Interface
3.4 A BackscatteringLight Detection Assembly for Sensitive Determination of Analyte
Concentrated at the Liquid/Liquid Interface Using the Interaction of Quercetin with Proteins as
the Model System
3.5 Flow-injection Resonance Light Scattering Detection of Proteins at the Nanogram Level
3.6 Resonance Light Scattering Imaging Detection of Proteins
with α,β,γ,δ-tetrakis (p-sulfophenyl) Porphyrin
Chapter 4 Analytical Applications in Organic Micromolecles and Medicines Detection of
Light Scattering Technique
4.i Enhanced Plasmon Resonance Light Scattering Signals of Colloidal Gold Resulted
from its Interactions with Organic Small Molecules Using Captopril as an Example
4.2 Total Internal Reflected Resonance Light Scattering Determination of Chlortetracycline
in Body Fluid with the Complex Cation of Chlortetracycline-europium-trioctyl Phosphine Oxide
at the water/tetrachloromethane interface
4.3 Novel Assay of Thiamine Based on its Enhancement of Total Internal Reflected Resonance
Light Scattering Signals of Sodium Dodecylbenzene Sulfonate at the Water/Tetrachloromethane
Interface
4.4 Adsorption of PeniciUin-berberine Ion Associates at a Water/Tetrachloromethane Interface
and Determination of Penicillin Based on Total Internal-reflected Resonance Light Scattering
Measurements
4.5 Pharmacokinetic Detection of Penicillin Excreted in Urine Using a Totally Internally
Reflected Resonance Light Scattering Technique with Cetyltrimethy
4.6 A Wide Dynamic Range Detection of Biopolymer Medicines with Resonance Light Scattering
and Absorption Ratiometry
4.7 A resonance Light Scattering Ratiometry Applied for Binding Study of Organic Small
Molecules with Biopolymer
4.8 A Light Scattering and Fluorescence Emission Coupled Ratiometry Using the Interaction
of Functional CdS Quantum Dots with Aminoglycoside Antibiotics as a Model System
4.9 Resonance Light Scattering Imaging Determination of Heparin
4.10 Visual Detection of Sudan Dyes Based on the Plasmon Resonance Light Scattering Signals
of Silver Nanoparticles